Pac5.1/v5-his b
http://www.biofeng.com/zaiti/kunchong/pAc5.1-V5-His-B.html WebThe pAC5.1/V5-His vectors can be used with the DES™-Inducible Kits (K512001 and K412001) for constitutive expression of your protein of interest. The pAc5.1/V5-His …
Pac5.1/v5-his b
Did you know?
WebThe lacZ reporter was generated by inserting a PCR fragment of the complete lacZ coding region (amplified from plasmid pAc5.1/V5-His/LacZ [Invitrogen]) in the KpnI and XhoI sites of pAc5.1. In the lacZ reporter the bovine growth hormone gene (BGH) poly(A) signal (PCR amplified from pCDNA3.1 [Invitrogen]) was inserted into the KpnI site ... WebKEYWORDS pAc5.1 V5-His B SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5379) AUTHORS Invitrogen (Life Technologies) TITLE …
Web3 Results for: pAc5.1/V5-His B 0 — 3 of 3. Name Vector Type Resistance Marker Bacterial Resistance Source Sequence Available ... WebpAC5.1/V5-His vectors are designed for use in Drosophila cells to achieve high-level transient expression of recombinant proteins. The vector offers the strong, constitutive …
WebThe pAC5.1/V5-His vectors can be used with the DES™-Inducible Kits (K512001 and K412001) for constitutive expression of your protein of interest. The pAc5.1/V5-His vectors also offer the following features: • Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency WebA biweekly scientific journal publishing high-quality research in molecular biology and genetics, cancer biology, biochemistry, and related fields
WebRatsupites Str. 1, 1067 Riga, Latvia3; and Robert-Koch-Institut, Nordufer 20, 13353 Berlin, Germany4 Received 31 May 2006/Returned for modification 9 July 2006/Accepted 28 September 2006 Human infections with Asian and European hantaviruses can result in hemorrhagic fever with renal
WebpAc5.1/V5-His A, B, and C Ac5 forward ACACAAAGCCGCTCCATCAG a-Factor TACTATTGCCAGCATTGCTGC Pichia methanolica expression vector AUG1 forward … facts about mount whitneyWebJul 1, 2015 · The pAC5.1-V5-His A was combined with pIE1-neo (Novagen, Cambridge MA, USA) to generate insect-cell-stable-expression vector, pAC5.1-neo. Sequentially, the trans-element of miRNA expression vector was amplified and inserted into pAC5.1-neo. A schematic process for construction of stable knockdown systems was shown in S2 Text. … facts about mouse spidersWebDec 12, 2024 · The Drosophila inducible expression system vector, pAc5.1/V5-His B (Invitrogen, Carlsbad, CA), was used to construct plasmid that express protein in Drosophila S2 cells. The diap1 or ntan1 ORF was amplified from fly cDNAs, kindly provided by Dr. Jianquan Ni (Tsinghua University, Beijing, China), by polymerase chain reaction (PCR). facts about mousehole cornwallWeb丁香通商铺武汉华尔纳生物科技有限公司为您提供细胞库 / 细胞培养、elisa 试剂盒、抗体、试剂、耗材等分类产品11件，全方位满足您的科研实验采购需求 do fish store foodWebA biweekly scientific journal publishing high-quality research in molecular biology and genetics, cancer biology, biochemistry, and related fields do fish sufferWebMultiple cloning site of pAc5.1/V5-His B Below is the multiple cloning site for pAc5.1/V5-His B. The start of transcription can occur at either nucleotide 2,478, 2,479, or 2,480. … facts about movie jawsWebJun 1, 2015 · The pAc5.1-GFP vector was reconstructed by cloning the GFP coding sequence into a Drosophila expression vector pAc5.1/V5-His B (Invitrogen) at BstBI/PmeI sites to replace the V5-His tag. The open reading frame (ORF) of LvJAK was cloned into the XbaI/SacII sites of pAc5.1/V5-His B and pAc5.1-GFP vectors for expressing V5-tagged or … do fish stop swimming to sleep