Lck and dhhc2
Web1 jan. 2024 · DHHC3/7 subfamily palmitoylates most of palmitoyl-proteins, such as PSD-95, GAP-43, Gα, GABA A Rγ2, and SNAP-25. DHHC9/18 subfamily (in purple) palmitoylates H-Ras, DHHC21 (in brown) palmitoylates Lck and eNOS, and DHHC17 (in yellow) palmitoylates SNAP-25 and huntingtin. Web1 feb. 2012 · DHHC2-dependent palmitoylation also has multiple functional effects. Firstly it promotes physical associations between CD9 and CD151, and between the α3 integrin …
Lck and dhhc2
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Web6 aug. 2010 · DHHC2 is, thus, a candidate enzyme to regulate SNAP25/23 palmitoylation dynamics at the plasma membrane. Finally, we demonstrate that overexpression of specific Golgi-localized DHHC proteins active against SNAP25/23 proteins perturbs the normal secretion of human growth hormone from PC12 cells. Publication types Research … Web1 feb. 2012 · DHHC2 was also found to be engaged in the regulation of SNAP25/23 palmitoylation dynamics at the plasma membrane ( Greaves et al., 2010 ). Moreover, some experiments have revealed that the nonreceptor tyrosine kinase Lck ( Jennings et al., 2009) and eNOS ( Fernandez-Hernando et al., 2006) were good substrates for DHHC2. …
Web31 okt. 2011 · Primary antibodies used were rabbit anti-DHHC2 (see below), 1:500 dilution, and mouse anti-Lck (3A5, Santa Cruz, 1:80 dilution) and secondary antibodies were … WebAlthough palmitoylation markedly affects tetraspanin protein biochemistry and functions, relevant palmitoylating enzymes were not known. There are 23 mammalian "DHHC" (Asp-His-His-Cys) proteins, which presumably palmitoylate different sets of protein substrates. Among DHHC proteins tested, DHHC2 bes …
Web2 aug. 2013 · DHHC2 and DHHC3 fused to Renilla luciferase or YFP retain PAT activity. HEK-293 cells were transfected with plasmids encoding DHHC2 (A) ... activity of the DHHC fusion proteins was confirmed by in vitro assays using an N-terminal fragment of N-myristoylated Lck as a substrate (Fig. 1, A and B, top panels). Web30 jan. 2008 · Our preliminary results suggest that DHHC21 favors the substrate with cysteines near myristoylated glycine, such as Lck, Fyn, and eNOS (Fukata et al., unpublished data). Mutagenesis analyses of residues surrounding palmitoylation sites and structural analyses will determine the exact consensus sequence of protein palmitoylation.
WebS-acyltransferase DHHC2 as an enzyme capable of palmitoylating of Lck in T cells. Reducing the DHHC2 level in Jurkat T cells using siRNA causes decreased Lck S …
Web21 mrt. 2024 · Identification of CKAP4/p63 as a substrate of DHHC2, a putative tumor suppressor. CK MNF116 and p63 were useful in identifying squamous cell carcinomas with single cell infiltration. Olomoucine, a CDK inhibitor, up-regulates CKAP4, a cytoskeleton-linking membrane protein. edwina boffey coventry ukWeb1 feb. 2009 · To test Lck as a substrate for DHHC2, we used a model substrate consisting of the N-terminal myristoylation, palmitoylation motif (SH4 domain) and the SH3 and SH2 … consumer services ontarioWeb7 feb. 2010 · Here we identify the DHHC family protein S-acyltransferase DHHC2 as an enzyme capable of palmitoylating of Lck in T cells. Reducing the DHHC2 level in Jurkat … edwin a. blumWebHere we identify the DHHC family protein S-acyltransferase DHHC2 as an enzyme capable of palmitoylating of Lck in T cells. Reducing the DHHC2 level in Jurkat T cells using siRNA causes decreased Lck S-acylation and partial dislocation from membranes, … consumer service specialistWeb13 jan. 2012 · Search worldwide, life-sciences literature Search. Advanced Search consumer services of madison countyWebDHHC2 is a protein S-acyl transferase for Lck (Short title: DHHC2 is a Lck PAT) Ruth Zeidman, Gemma Buckland, Marek Cebecauer, Philipp Eissmann*, Daniel M. Davis * and Anthony I. Magee. edwina booth titanicWeb1 jun. 2011 · The DHHC family protein S-acyltransferase DHHC2 is identified as an enzyme capable of palmitoylating of Lck in T cells and localizes primarily to the endoplasmic reticulum and Golgi apparatus suggesting that it is involved in S-ACYlation of newly-synthesized or recycling Lck involved in T cell signalling. 15 View 2 excerpts, cites … edwina booth pics