2024 Fastpfu polymerase

Fastpfu polymerase

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August undefined, 2024

WebTransStart ® FastPfu Fly DNA Polymerase is a hot start, high-fidelity and high processivity DNA Polymerase used for fast PCR. Compared with TransStart ® FastPfu DNA Polymerase, TransStart ® FastPfu Fly … WebTransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase. • TransStart® FastPfu DNA Polymerase offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase. • PCR products can be directly cloned into …

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WebCharacteristics. - TransStart® FastPfu Fly DNA Polymerase offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase. - PCR products can be directly cloned into … WebDec 26, 2024 · PCR扩增采用TransStart Fastpfu DNA Polymerase，20 μL反应体系：5×FastPfu Buffer 4 μL，2.5 mmol/L dNTPs 2 μL，5 μmol/L上下游引物338F (5′-ACT CCTACGGGAGGCAGCAG-3′)和806R (5′-GGA CTACHVGGGTWTCTAAT-3′)各0.8 μL，FastPfu Polymerase 0.4 μL，BSA 0.2 μL，模板DNA 10 ng，补充ddH 2 O至20 μL。 shoba cherian

Pfu DNA Polymerase - Promega

WebTransStart ® FastPfu DNA polymerase (FastPfu), TransStart ® FastPfu Fly DNA polymerase (FastPfu Fly) and EasyPfu DNA Polymerase (EasyPfu) were purchased from TransGen Biotech. Proteinase K from Tritirachium album limber and elastase from the porcine pancreas were purchased from Roche (Mannheim, Germany) and Sangon … WebJul 5, 2024 · PCR reactions were performed in a 20 μL mixture containing 4 μL of ×5 FastPfu Buffer, 2 μL of 2.5 mm dNTPs, 0.8 μL of each primer (5 μM), 0.4 μL of FastPfu Polymerase, and 10 ng of template DNA. ... (5 μM), 0.4 μL of FastPfu Polymerase, and 10 ng of template DNA. EmPCR products were prepared using the Roche emPCRAmp … WebAug 23, 2024 · The PCR mixtures contain 5 × TransStart FastPfu buffer 4 μL, 2.5 mM dNTPs 2 μL , forward primer (5 μM) 0.8 μL, reverse primer (5 μM) 0.8 μL, TransStart FastPfu DNA Polymerase 0.4 μL, template... shoba chennai

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WebFeb 8, 2016 · TransStart FastPfu Fly DNA Polymerase is a hotstart, ultra-high fidelity and high-processivity DNA Polymerase. It consists in a modified DNA Polymerase, increasing its affinity for dsDNA and … WebPhusion ® High-Fidelity DNA Polymerase For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific ® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance. 50X higher fidelity than Taq Robust reactions - maximal success with minimal optimization shoba chandrasekhar vidhya chandrasekharWebThe PCR reactions were performed in triplicate 20 μL of mixtures containing 0.4 μL of FastPfu polymerase, 4 μL of 5 × FastPfu buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of each primer (5 μM), and 10 ng of template DNA. The amplicons were purified from 2% (w/v) agarose gels using an AxyPrep DNA gel extraction kit (Axygen Biosciences, Union City ... rabbits foot bethpage

"WebTransStart® FastPfu Fly DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of TransStart® FastPfu Fly DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human … " - Fastpfu polymerase

Fastpfu polymerase

WebMar 8, 2024 · For each sample, PCR reactions for bacteria were carried out in triplicate 20-μL reactions with 0.4 μL of each primer at 5 μmol⋅L −1, 10 ng template DNA, 2 μL dNTPs at 2.5 mmol⋅L −1, 0.4 μL FastPfu Polymerase (TransGen AP221-02: TransStart FastPfu DNA Polymerase; TransGen Biotech), 4 μL 5× FastPfu buffer, and certified DNA-free ... WebDec 23, 2024 · Based on polymerase chain reaction (PCR) method and 16S rRNA sequence similarity values, ... 0.8 μL of forward and reverse primers (5 μM), 0.4 μL of FastPfu Polymerase and 10 ng of template DNA were for PCR amplification in triplicate. PCR amplicons were purified by AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, …

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Web· Select high-fidelity DNA polymerases: Family B DNA polymerase, such as Pfu DNA polymerase, with 3′→ 5′exonuclease activity, corrects mismatched base pairs to ensure high fidelity. In addition, polymerase blends containing Family B DNA polymerases also have a high fidelity, which is lower than the fidelity of Family B DNA polymerase. WebSep 18, 2024 · The formal PCR test used TransGen AP221-02: TransStart Fastpfu DNA Polymerase, 20 μl reaction system: 5×FastPfu buffer 4 μl, 2.5 mM dNTPs 2 μl, forward primer (5 μM) 0.8 μl, reverse primer (5 μM) 0.8 μl, FastPfu polymerase 0.4 μl, BSA 0.2 μl, template DNA 10 ng, and supplement ddH 2 O to 20 μl. The following thermal cycling …

http://school.freekaoyan.com/bj/wswyjs/2024/12-26/16405189191500959.shtml WebJul 22, 2024 · PCR amplification was performed using the TransStart ® FastPfu Polymerase system (Transgen Biotech, Beijing, China), including 4 μL 5 × FastPfu Buffer, 2 μL dNTPs (2.5 mM), 0.8 μL each primer (5 μM), 0.4 μL FastPfu Polymerase, and 10 ng template DNA. The reaction mixture was initially denatured at 95°C for 2 min, followed by …

WebPfu Polymerase is a thermostable DNA polymerase isolated from the archaeal thermophile Pyrococcus furiosus. The 90-kDa Pfu polymerase enzyme features a 3’-5’ exonuclease … WebJun 16, 2024 · TransGen AP221 kit with TransStart Fastpfu DNA polymerase was used for PCR. PCR was carried out in 20 μl reactions including 5 × FastPfu buffer 4 μl, 2.5 mM dNTPs 2 μl, each primer (5 μM) 0.8 μl, template DNA 10 ng, FastPfu polymerase (Transgen Biotech, Beijing, China) 0.4 μl, and replenishment ddH2O to 20 μl.

WebPfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in …

WebApr 28, 2024 · The raw materials used for PCR reaction were as follows: Transgen AP221–02:Transstart FastPFU DNA Polymerase, 20μL reaction system: 5 × TransStart FastPfu buffer (4μL), 2.5 mM dNTPs (2μL), forward primer (5μM) 0.8μL, reverse primer (5μM) 0.8μL, TransStart FastPfu polymerase 0.4μL,BSA 0.2μL, template DNA 10 ng, … shoba companyWebTransgen Ap221 02 Transstart Fastpfu Dna Polymerase, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. … shoba cherian nanuet nyWebTransStart® FastPfu Fly DNA Polymerase is a hot start, high fidelity and high processivity DNA Polymerase. TransStart® FastPfu Fly DNA Polymerase. sho badarpur police stationWebApr 11, 2024 · PCR reactions were performed in quadruplicate using a 20 μL mixture consisting of 4 μL of 5× TransStart FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of Forward Primer and Reverse Primer (5 μM), 0.4 μL of FastPfu Polymerase, 0.2 μL BSA, 10 ng Template DNA, and supplemented with sterile water to 20 μL. The qPCR product was … shobac point houseWebAll the polymerase chain reaction (PCR) processes used a 20 μL TransStart Fastpfu DNA polymerase reaction system, which included 4 μL 5× FastPfu buffer, 2 μL 2.5 mmol/L dNTPs, 0.8 μL 5... shoba clan namesWebMar 30, 2024 · The PCR mixtures contain 4 μL of 5 × TransStart FastPfu buffer, 2.5 mM dNTPs 2 μL, forward primer (5 μM) 0.8 μL, reverse primer (5 μM) 0.8 μL, TransStart FastPfu DNA Polymerase 0.4 μL, template DNA 10 ng, and finally ddH2O up to 20 μL. The PCR reactions were carried out in triplicate. shoba cherian nanuetWeb本发明公开一种基于环境DNA技术监测淡水底栖动物群落多样性的方法，包括步骤：(1)采集表层水样；(2)水样过滤与eDNA提取；(3)PCR扩增；(4)高通量测序，获得OTU的代表序列；(5)底栖动物比对数据库的建立；(6)比对建立的底栖动物数据库，对待测样品OTUs代表性序列进行物种注释。本发明取样简单、人力 ... shoba dawson bristol