WebJul 3, 2007 · This three-hybrid technology allows two designated cellular proteins to be brought into proximity and has been used to control many processes, including transcription and translocation (8), receptor signaling (9), and protein splicing (10). WebAug 28, 2015 · The degron peptide sequence is located within the previously characterized incompletely folded C-terminus of IκBα , and deviations from the AR consensus were noted. Indeed, mutations, Y254L and T257A towards the AR consensus increased IκBα foldedness and in vivo half-life ( 9 ).
The Eukaryotic Proteome Is Shaped by E3 Ubiquitin …
WebC-terminal degron discovery. It has long been known that the stability of proteins is influenced by their N-terminal residue, and a large body of work over the past three decades has characterized a collection of N-end rule pathways that target proteins for degradation through N-terminal degron motifs. Whether equivalent pathways exist to ... WebOct 20, 2024 · A combined structural and biochemical analysis reveals that TRIM7 E3 ligase targets viral proteins for degradation by recognizing their C-terminal glutamine (C-Gln) … buffy the vampire slayer trivia
Molecular basis for arginine C-terminal degron ... - ResearchGate
WebNov 1, 2024 · Cereblon (CRBN) is a ubiquitously expressed E3 ligase substrate receptor and a key player in pharmaceutical targeted protein degradation. Despite substantial … WebDec 19, 2024 · The SsrA C-degron is particularly useful because its sequence can be easily identified within the tmRNA of different species. Since this degron is only functional when exposed at the C-terminus (Wei et al., 2011), all of the current targeted proteolysis systems are limited to C-terminal tagging. This may be problematic for some proteins if, for ... WebJun 29, 2024 · Since degrons are normally used to regulate protein expression and activity in cells [ 33, 34 ], we use bioinformatic and experimental approaches to better … buffy the vampire slayer t shirt primark